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Image Search Results
Journal: Bone
Article Title: Preservation of Type H Vessels and Osteoblasts by Enhanced Preosteoclast Platelet-Derived Growth Factor type BB Attenuates Glucocorticoid-Induced Osteoporosis in Growing Mice
doi: 10.1016/j.bone.2018.05.025
Figure Lengend Snippet: (A-B) Representative images of immunofluorescence staining of platelet-derived growth factor type BB (PDGF-BB) (green) and tartrate-resistant acid phosphatase (TRAP) (red) with quantification of number of PDGF-BB+ TRAP+ cells (yellow) in femoral primary (A) and secondary spongiosa (B) of 6 week old Ctsk+/+ wild type (WT) and Ctsk−/− mice injected with either vehicle (veh) or prednisolone 10 mg/m2/day (pred) for 4 weeks. DAPI stains nuclei blue. Scale bar, 50 μm. (C) Bone marrow PDGF-BB concentration as analyzed by ELISA. n = 6 per group. Data shown as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet: We performed PDGF-BB ELISA analysis of bone marrow supernatant using a
Techniques: Immunofluorescence, Staining, Derivative Assay, Injection, Concentration Assay, Enzyme-linked Immunosorbent Assay